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axio observer 7 inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axio observer 7 inverted fluorescence microscope
    Axio Observer 7 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 991 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+fluorescence+microscope/Inverted+microscope+Axio+Observer+7/pmc13657243-228-6-5
    Average 98 stars, based on 991 article reviews
    axio observer 7 inverted fluorescence microscope - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: Chromosome-scale assembly of yeast genomes using enzyme-free, dense optical genome mapping.
    Article Snippet: .. Stretched and stained DNA molecules in the nanochannels were then imaged by using an inverted fluorescence microscope (Zeiss Axio Observer D.1) equipped with a 63x oil immersion objective (NA = 1.46, Zeiss), a Colibri 7 light source (Zeiss), and a Photometrics Evolve EMCCD camera. .. Systematic acquisition of videos of up to 100 frames was carriedout for eachmolecule, utilizing anexposure timeof 100msand a FITC filter set (Zeiss).

    Article Title: Soft, curved and transparent fish scale biocornea for anterior lamellar keratoplasty.
    Article Snippet: Due to structural and compositional similarities with human corneas, decellularized fish scale scaffolds have emerged as potential substitutes for donor corneas.. However, to date, their effective use in anterior lamellar keratoplasty has rarely been reported, as their rigid texture and poor conformity to the curved corneal bed can cause graft–host mismatch and sustained mechanical irritation.. To overcome these limitations, this study introduced a softening strategy to convert rigid decellularized fish scales into soft and curved matrices.

    Incubation:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Fluorescence:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: Photoaging dynamics of tire wear particles in the terrestrial-aquatic interface: The crucial role of soil solution chemistry and dissolved organic matter evolution.
    Article Snippet: Tire wear particles (TWPs) represent one of the primary sources of microplastic pollution entering aquatic systems via road runoff and soil leaching.. Once deposited at the terrestrial-aquatic interface, the inevitable photoaging of TWPs in soil solutions dictates their subsequent environmental fate and toxicological profile.. This study investigates the time-dependent photoaging behavior of TWPs within soil solutions derived from three representative soil types: brown soil (BS), paddy soil (PS), and fluvo-aquic soil (FS).

    Article Title: Silver Nitrate‐Doped Soft Silicone Foam Dressing for Skin Wound Healing
    Article Snippet: Soft silicone dressings have been on the market for more than a decade.. They were created to protect the skin around the wound, reduce pain and stress during dressing changes, and enhance comfort while being worn.. Silver nitrate (SN)doped silicone foam was successfully fabricated by mixing silicone solution with the SN solution.

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: Red fluorescent nanoparticles (excitation/emission: 580/605 nm; Sigma-Aldrich) were dispersed into the hydrogel precursor (1% v/v) via vortexing and sonication prior to standard fiber fabrication. .. To assess nanoparticle distribution, transverse fiber sections were imaged using an inverted fluorescence microscope (Axio Observer, Carl Zeiss). .. For SEM analysis, fabricated fibers were dehydrated in a graded ethanol series (70–100% v/v, 30 min/step) and critical-point dried (CPD300, Leica).

    Article Title: Chromosome-scale assembly of yeast genomes using enzyme-free, dense optical genome mapping.
    Article Snippet: .. Stretched and stained DNA molecules in the nanochannels were then imaged by using an inverted fluorescence microscope (Zeiss Axio Observer D.1) equipped with a 63x oil immersion objective (NA = 1.46, Zeiss), a Colibri 7 light source (Zeiss), and a Photometrics Evolve EMCCD camera. .. Systematic acquisition of videos of up to 100 frames was carriedout for eachmolecule, utilizing anexposure timeof 100msand a FITC filter set (Zeiss).

    Article Title: A concave corneal stromal scaffold based on multi-crosslinked GelMA hydrogel for delaying keratoconus progression and improving corneal morphology.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Gut microbiota primes stroke severity via the AHR in intestinal dendritic cells.
    Article Snippet: Tissue sections (8 μm) were counterstained (1:1000 dilution) with DAPI to detect cell nuclei and phalloidin i-Fluor 647 (Abcam, ab176759) to detect actin filaments. .. At least 60 pictures per mouse were acquired from 3 consecutive intestinal sections with an inverted fluorescence microscope (Zeiss Axio Observer Z1, 20x magnification). ..

    Article Title: Soft, curved and transparent fish scale biocornea for anterior lamellar keratoplasty.
    Article Snippet: Due to structural and compositional similarities with human corneas, decellularized fish scale scaffolds have emerged as potential substitutes for donor corneas.. However, to date, their effective use in anterior lamellar keratoplasty has rarely been reported, as their rigid texture and poor conformity to the curved corneal bed can cause graft–host mismatch and sustained mechanical irritation.. To overcome these limitations, this study introduced a softening strategy to convert rigid decellularized fish scales into soft and curved matrices.

    Microscopy:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: Photoaging dynamics of tire wear particles in the terrestrial-aquatic interface: The crucial role of soil solution chemistry and dissolved organic matter evolution.
    Article Snippet: Tire wear particles (TWPs) represent one of the primary sources of microplastic pollution entering aquatic systems via road runoff and soil leaching.. Once deposited at the terrestrial-aquatic interface, the inevitable photoaging of TWPs in soil solutions dictates their subsequent environmental fate and toxicological profile.. This study investigates the time-dependent photoaging behavior of TWPs within soil solutions derived from three representative soil types: brown soil (BS), paddy soil (PS), and fluvo-aquic soil (FS).

    Article Title: Silver Nitrate‐Doped Soft Silicone Foam Dressing for Skin Wound Healing
    Article Snippet: Soft silicone dressings have been on the market for more than a decade.. They were created to protect the skin around the wound, reduce pain and stress during dressing changes, and enhance comfort while being worn.. Silver nitrate (SN)doped silicone foam was successfully fabricated by mixing silicone solution with the SN solution.

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: Red fluorescent nanoparticles (excitation/emission: 580/605 nm; Sigma-Aldrich) were dispersed into the hydrogel precursor (1% v/v) via vortexing and sonication prior to standard fiber fabrication. .. To assess nanoparticle distribution, transverse fiber sections were imaged using an inverted fluorescence microscope (Axio Observer, Carl Zeiss). .. For SEM analysis, fabricated fibers were dehydrated in a graded ethanol series (70–100% v/v, 30 min/step) and critical-point dried (CPD300, Leica).

    Article Title: Chromosome-scale assembly of yeast genomes using enzyme-free, dense optical genome mapping.
    Article Snippet: .. Stretched and stained DNA molecules in the nanochannels were then imaged by using an inverted fluorescence microscope (Zeiss Axio Observer D.1) equipped with a 63x oil immersion objective (NA = 1.46, Zeiss), a Colibri 7 light source (Zeiss), and a Photometrics Evolve EMCCD camera. .. Systematic acquisition of videos of up to 100 frames was carriedout for eachmolecule, utilizing anexposure timeof 100msand a FITC filter set (Zeiss).

    Article Title: A concave corneal stromal scaffold based on multi-crosslinked GelMA hydrogel for delaying keratoconus progression and improving corneal morphology.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Gut microbiota primes stroke severity via the AHR in intestinal dendritic cells.
    Article Snippet: Tissue sections (8 μm) were counterstained (1:1000 dilution) with DAPI to detect cell nuclei and phalloidin i-Fluor 647 (Abcam, ab176759) to detect actin filaments. .. At least 60 pictures per mouse were acquired from 3 consecutive intestinal sections with an inverted fluorescence microscope (Zeiss Axio Observer Z1, 20x magnification). ..

    Article Title: Soft, curved and transparent fish scale biocornea for anterior lamellar keratoplasty.
    Article Snippet: Due to structural and compositional similarities with human corneas, decellularized fish scale scaffolds have emerged as potential substitutes for donor corneas.. However, to date, their effective use in anterior lamellar keratoplasty has rarely been reported, as their rigid texture and poor conformity to the curved corneal bed can cause graft–host mismatch and sustained mechanical irritation.. To overcome these limitations, this study introduced a softening strategy to convert rigid decellularized fish scales into soft and curved matrices.



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